Figure 7 | Cell Death & Disease

Figure 7

From: Cdc20 directs proteasome-mediated degradation of the tumor suppressor SMAR1 in higher grades of cancer through the anaphase promoting complex

Figure 7

Cdc20 impairs the tumor suppressive function of SMAR1 in higher grade of breast cancer. (a) Depletion of Cdc20 suppresses growth of MDA-MD-231. Long term survival assay (Colony formation assay) showed the colony formation ability of MDA-MB-231 cells expressing either scramble shRNA or shCdc20 or coexpression of both shCdc20 and shSMAR1. (b) Soft agar analysis assay showed the anchorage independent growth potential of MDA-MB-231 cells expressing either scramble shRNA or shCdc20 or coexpression of both shCdc20 and shSMAR1. (c) Quantification of colonies from soft agar assay. An asterisk indicates the significant difference (*P<0.05) calculated by one way ANOVA test. (d) Cell migration potential of MDA-MB-231 cells expressing either scramble shRNA or shCdc20 or coexpression of shCdc20 and shSMAR1. Quantification of migration data were presented graphically in the lower panel. An asterisk indicates the significant difference (*P<0.05) calculated by one way ANOVA test. (e) Graphical representation of IC50 value for doxorubicin in MDA-MB-231 cells upon stable knockdown of Cdc20 and Cdc20 and SMAR1. An asterisk indicates the significant difference (*P<0.05) calculated by one-way ANOVA test

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