Figure 3

LBL21 caused ROS increase and GSH depletion. (a) DCF-DA staining showed the effect of PEITC (green line) and LBL21 (red line) at 10 μM on cellular ROS levels after 6 h treatment. (band c) Pretreatment with NAC at 3 mM effectively reversed the ROS accumulation (6 h) and apoptosis rates (48 h) induced by LBL21 at 10 μM. Percentage numbers indicate cell death population. (d) Cellular GSH contents after 10 μM LBL21 or PEITC treatment for indicated hours. Bars means ±S.D. *P<0.05, **P<0.01 versus control, n=3