Figure 6 | Cell Death & Disease

Figure 6

From: Loss of the novel mitochondrial protein FAM210B promotes metastasis via PDK4-dependent metabolic reprogramming

Figure 6

FAM210B knockdown cells show decreased PDK4 expression. (a) Heat map of the top 25 differentially regulated genes in siFAM210B and negative control A549 cells. (b) Quantitative PCR analyses of the relative expression of the indicated genes in siFAM210B normalized to negative control SKOV3 cells. (c) Immunoblotting for PDK4 in the indicated treated SKOV3 cells. (d) Schematically depicted PDK function in SKOV3 cell metabolism. (e) Western blotting analysis of the phosphorylation levels of PDH-E1α in the indicated treated SKOV3 cells. (f) Normalized OCR and (g) normalized ECAR in siPDK4 (n=6) and negative control SKOV3 cells (n=8 wells)

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