Figure 6
From: Heme oxygenase-1 derived carbon monoxide suppresses Aβ1–42 toxicity in astrocytes
HO-1 induction protects astrocytes from Aβ1–42 toxicity via CO formation. (a) Left, western blot for HO-1 taken from control astrocytes and astrocytes exposed to CoPPIX (3 μM) for 24 h, as indicated. β-actin was also probed to confirm approximately equal protein loading of lanes. Below, mean±S.E.M. (n=3) relative densitometric readings for control and CoPPIX-treated cells, as indicated. *P<0.05. Right, images of control and CoPPIX-treated cells, immunostained for HO-1. Scale bar applied to both images. (b) Effect on cell viability of a 24 h exposure of astrocytes to Aβ1–42 alone (100 nM, white bar) or Aβ1–42 in the additional presence of 3 μM CoPPIX. Also shown is the lack of effect of CoPPIX alone. Bars represent the mean±S.E.M. data of cells from 5 repeats (each performed in duplicate) with cells from different preparations. ***P<0.001. (c) Effect on cell viability of a 24 h exposure of astrocytes to 100 nM Aβ1–42 in the absence (white bar) or presence (dark grey bars) of the CO donor CORM-2 (3–20 μM). The effects of CORM-2 alone are also presented (light grey bars), along with the effects of 100 nM Aβ1–42 in the additional presence of the inactive form of CORM-2, iCORM (hatched bars). Bars represent the mean±S.E.M. data of cells from 6 repeats (each performed in duplicate) with cells from different preparations. ***P<0.001
