Figure 2 | Cell Death & Disease

Figure 2

From: Reduced brain UCP2 expression mediated by microRNA-503 contributes to increased stroke susceptibility in the high-salt fed stroke-prone spontaneously hypertensive rat

Figure 2

Characterization of NF-κB protein expression and of oxidative stress level in brains of JD-fed SHRSR and SHRSP (4 weeks of dietary regimen). (a) WB of NF-κB expression with corresponding densitometric analysis in SHRSR upon RD or JD. (b) WB of carbonylated total proteins in SHRSR upon RD or JD. Each lane was loaded with 50 μg of total proteins. Lane M, DNP marker. Each sample was run with its own untreated control (C). Normalization for lane protein loading was performed using Coomassie staining. The corresponding densitometric analysis is shown on the right side of the panel. Bar graphs represent chemiluminescence intensity relative to the gel loading band. Bands 1 to 5 refer to the most prominent bands on the blots (identified by arrows), whereas total refers to the total chemiluminescence intensity from all bands. (c) WB of NF-κB expression with corresponding densitometric analysis in SHRSP upon RD or JD. ***P<0.0001 for JD versus RD. (d) WB of carbonylated total proteins in SHRSP upon RD or JD with corresponding densitometric analysis shown on the right side of the panel. See legend of panel (b). ***P<0.0001 for JD versus RD

Back to article page