Figure 2 | Cell Death & Disease

Figure 2

From: Protein kinase A activation by the anti-cancer drugs ABT-737 and thymoquinone is caspase-3-dependent and correlates with platelet inhibition and apoptosis

Figure 2

PKA and PKG activation inhibits Thr/Cvx-induced platelet activation and procoagulant activity. Washed human platelets (1 × 107/ml) were treated with a combination of thrombin (Thr, 0.005 U/ml) and convulxin (Cvx, 5 ng/ml) for 2 min without (ø) or with DEA-NO (1 μM, 2 min) or forskolin (5 μM, 2 min) preincubation. (a) PKA/PKG activity was monitored using western blot analysis of VASP Ser239-phosphorylation (P-VASP239). Actin served as loading control. The presented western blot is representative of five independent experiments. (b–e) FACS analysis of integrin αIIbβ3 activation (PAC-1 binding (b), P-selectin exposure (c), ΔΨm (d), and PS-exposure (Annexin V binding (e). Data in (b–e) are fold increase of mean fluorescence intensity (MFI) compared to control (considered as 1) and are presented as mean±S.E.M., n=5; *P<0.05 compared to Thr/Cvx-stimulated platelets without DEA-NO or forskolin preincubation (ø)

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