Figure 4 | Cell Death & Disease

Figure 4

From: Protein kinase A activation by the anti-cancer drugs ABT-737 and thymoquinone is caspase-3-dependent and correlates with platelet inhibition and apoptosis

Figure 4

ABT-737 and TQ induce VASP phosphorylation in platelets. (a) Washed human platelets (3 × 108/ml) were stimulated with ABT-737 (1 μM, 60 min) or TQ (40 μM, 10 min), or in combination with DEA-NO (1 μM) or forskolin (5 μM) for the same time. (b) Platelets were stimulated with the same concentrations (as in a) of ABT-737 and DEA-NO, or forskolin for indicated time. (c) Platelets were stimulated by TQ for indicated time or with DEA-NO (1 μM), or forskolin (5 μM) for 2 min. PKA/PKG activity was monitored using western blot analysis of VASP Ser239-phosphorylation (P-VASP239). Actin served as loading control. Presented western blot results are representative of five independent experiments

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