Figure 6 | Cell Death & Disease

Figure 6

From: Protein kinase A activation by the anti-cancer drugs ABT-737 and thymoquinone is caspase-3-dependent and correlates with platelet inhibition and apoptosis

Figure 6

Impact of ABT-737- and TQ-treatment on platelet viability and integrin αIIbβ3 surface expression. Washed human platelets (1 × 108/ml) were treated with ABT-737 (1 μM) or TQ (40 μM) for the indicated times and analysed by FACS for viability (calcein-fluorescence) and integrin αIIbβ3 surface expression (CD41). All platelets were separated into three gates: gate A represents more than 93% calcein- and CD41-positive platelets in control samples, gate C corresponds to unstained platelets (calcein-negative/dead cells; or CD-41-negative platelets; compare (a)) and gate B represents platelets with intermediate calcein- or CD41 fluorescence. Distribution of platelets in each gate was calculated as a percent of all analysed (50 000) events taken as 100%. Data are presented as mean±S.E.M., n=4. (a–c) represent a set of original FACS data representative of four independent experiments

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