Figure 1 | Cell Death & Disease

Figure 1

From: RETRACTED ARTICLE: mTORC2 regulates hedgehog pathway activity by promoting stability to Gli2 protein and its nuclear translocation

Figure 1

Status of hedgehog pathway molecules in U87MG and LN229 cells. (a) Hypothesis: Is there any role of mTORC2 in regulating hedgehog pathway using GBM as a model system? (b) A representative immunoblot analysis (from three experiments) showing the status of the Gli1, Gli2FL, Gli3Rep, Ptch1, Sufu, Shh and Smo protein in U87MG and LN229 cells. β-Actin served as a loading control. For development of bands, Super Signal West Pico imaging system (Thermo-scientific) was used. (c) Fold change in mRNA expression of Gli1, Gli2, Gli3 and Ptch1 by real-time PCR analysis in LN229 cells relative to that of U87MG. Values are normalized against 18S rRNA expression (n=3 experiments). The data are presented as means ± SD of three independent experiments. Statistical significance compared with the control is indicated by **P<0.01 and ***P<0.001

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