Figure 2
From: miR-181d and c-myc-mediated inhibition of CRY2 and FBXL3 reprograms metabolism in colorectal cancer
Knockdown of miR-181d inhibits the proliferation, colony formation, and metastasis of CRC cells in vitro and in vivo. (a) Effective knockdown of miR-181d in CRC cells was confirmed by qRT-PCR. (b) After miR-181d knockdown, proliferation of CRC cells was measured by CCK8 assay. (c) Colony formation ability of CRC cells was suppressed once silencing miR-181d. (d) Analysis of the xenograft tumors formed by CRC cells with or without miR-181d knockdown (first and second panel). CRY2, FBXL3, c-myc, HDAC3 (third panel), and miR-181d levels (fourth panel) were presented. 6 S rRNA and actin were used as internal controls of qRT-PCR and western blotting, respectively. (e) Migration and invasion assays of CRC cells after miR-181d knockdown. (f) Assay of lung metastatic foci number after tail vein injection and representative images
