Figure 6
From: miR-181d and c-myc-mediated inhibition of CRY2 and FBXL3 reprograms metabolism in colorectal cancer
FBXL3 and CRY2 are direct targets of miR-181d. (a) qRT- PCR analysis of FBXL3 and CRY2 mRNA expression. (b) The expression of FBXL3 and CRY2 was analyzed using western blot in the indicated cells. (c) Predicted miR-181d target sequences in the 3′-UTRs of FBXL3 and CRY2, and their mutations were generated in the 3′-UTR sequences at the complementary sites for the seed regions in miR-181d. (d) Luciferase reporter assays of the indicated cells. (e,f) Luciferase assays with the endogenous levels of miR-181d in HCT116 and SW620 cell lines. (g,h) The effects of FBXL3 Knockdown and c-myc overexpression on miR-181d-induced glycolysis in CRC cells
