Figure 1 | Cell Death & Disease

Figure 1

From: Pre-synaptic TrkB in basolateral amygdala neurons mediates BDNF signaling transmission in memory extinction

Figure 1

CC1-EGFP interrupted the TrkB-JIP3 interaction. (a) Schematic diagram of the domains of the JIP3 protein. CC1 was linked to EGFP to form a novel protein, CC1-EGFP. (b) HEK293 cells were co-transfected with FLAG-TrkB and CC1-EGFP. Immunoprecipitation was performed with the anti-FLAG antibody. Immunoblotting was performed with anti-Flag or anti-GFP antibodies, and the results showed that CC1-EGFP interacted with Flag-TrkB. The figure represents three independent experiments that yield similar result. (c,d) HEK293 cells were co-transfected with Myc-JIP3, FLAG-TrkB and CC1-EGFP. Lysates were immunoprecipitated with the anti-FLAG antibody. Immunoblotting showed that CC1-EGFP blocked the TrkB-JIP3 interaction in a dose-dependent manner. Data are shown as the mean±S.E.M. from three independent experiments (n=3, **P<0.01 compared to the Flag-TrkB-positive and CC1-EGFP-negative group). (e) CC1-EGFP interrupted the endogenous interaction of JIP3 with TrkB. Neurons infected with EGFP or CC1-EGFP-containing lentivirus were lysed and subjected to immunoprecipitation with an anti-JIP3 antibody. TrkB, JIP3 and GFP were detected by immunoblotting. The figure represents three independent experiments that yield similar result

Back to article page