Figure 5
CC1-EGFP overexpression in BLA neurons. (a) Construction of the CamkIIα promoter-driven CC1-EGFP in an AAV5 virus vector. (b) The localization and diffusion scope of the AAV5 virus in the BLA; scale bar, 100 μm. (c) CC1-EGFP was predominately expressed in CamkIIα-positive cells and rarely expressed in GAD67-positive cells; scale bar, 50 μm. (d) CC1-EGFP interrupted the endogenous interaction of JIP3 with TrkB. BLA neurons infected with EGFP or CC1-EGFP-containing AAV5. After that, BLA tissue were lysed and subjected to immunoprecipitation with anti-JIP3 antibody. TrkB, JIP3 and GFP were detected by immunoblotting. The figure represents three independent experiments that yield similar result. (e) CC1-EGFP decreased the distribution of TrkB at the axonal tips of BLA neurons. Rats BLA region were injected with AAV5-CKII-TrkB-mCherry which mixed with AAV5-CKII-EGFP or AAV5-CKII-CC1-EGFP. Green staining was the terminal of BLA neurons, and red represented exogenous TrkB. Pictures showed the IL region of rats. Scale bar, 10 μm. (n=4 per group)
