Figure 6
Expression of CC1-EGFP in the BLA impaired the extinction of fear memory. (a) Schematic diagram of the behavioral protocol. Rats received bilateral microinjection of EGFP or CC1-EGFP-containing AAV5 virus 24 h after cued fear conditioning training, and extinction training was performed 14 days later. (b–d) Freezing responses of rats during fear memory acquisition training, extinction training and testing. Data are shown as the mean±S.E.M. (EGFP group n=8, CC1-EGFP group n=9, *P<0.05, **P<0.01 compared to the EGFP group). (e,f) Phosphorylation levels of TrkB in the BLA and IL 2 h after extinction training. Lysates of the BLA and IL were subjected to immunoprecipitation with the anti-TrkB antibody and were immunoblotted with the pY99 antibody. Densitometric analysis showed the relative levels of p-TrkB compared to total TrkB from the immunoprecipitation. Data are shown as the mean±S.E.M. (n=3 per group, *P<0.05, **P<0.01 compared to the EGFP-infected no extinction group; #P<0.05, compared to the EGFP-infected extinction group)
