Figure 2 | Cell Death & Disease

Figure 2

From: Ubiquitin-dependent regulation of Cdc42 by XIAP

Figure 2

EGF treatment further enhances the stability of Cdc42 upon XIAP depletion (a) HeLa cells and (b) HMECs were transfected with XIAP siRNA and treated with EGF 48 h post transfection for the times indicated in the figure. The levels of Cdc42 were monitored via immunoblotting. (c, d) Active Cdc42 pulldown using GST PAK-PBD protein was performed in HeLa and HMECs to test for levels of the active protein upon EGF treatment at times indicated in the figure. * denotes a higher exposure of Cdc42 in (d). **denotes the upper non-specific band in the XIAP blot

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