Figure 6
From: p75NTR antagonists attenuate photoreceptor cell loss in murine models of retinitis pigmentosa
In vivo effect of the p75NTR antagonist THX-B in the rd10 retina. P17 rd10 mice were intravitreally injected with THX-B in one eye and with vehicle in the contralateral eye, and analyzed at P22. (a) Representative sections. Nuclei are stained with DAPI. (b) Comparison of the photoreceptor number per row between the THX-B-treated eye and the control eye. Paired eyes are individually represented. (c) Comparison of the ONL/INL thickness ratio between the THX-B-treated eye and the control eye. Paired eyes are individually represented. (d) Quantification of the microglial (TL-positive) cell density in the THX-B treated retina versus the control retina from the same animal. Paired eyes are individually represented. (e) RT-qPCR of THX-B-treated and control retinas. The levels of the different transcripts were normalized to the TBP RNA levels and relativized to control retina levels. Bars represent mean±S.E.M.; n≥3; *P<0.05, **P<0.01; two-way analysis of variance (ANOVA) for RT-qPCR of treated versus control retinas, F=4.40, P=0.0467. Scale bar: 20 μm. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; TL, tomato lectin
