Figure 7
From: p75NTR antagonists attenuate photoreceptor cell loss in murine models of retinitis pigmentosa
Effect of the P75NTR antagonist THX-B, both ex vivo and in vivo, in the RhoP retina. (a) P18 RhoP retinas were cultured for 24 h in the absence (vehicle) or presence of the THX-B. Photoreceptor cell death was visualized by TUNEL and quantified. (b) P17 RhoP mice were intravitreally injected with THX-B in one eye and with vehicle in the contralateral eye, and analyzed at P22. Nuclei in whole-mount retinas were counterstained with DAPI. Representative cryosections. (c) Comparison of the ONL/INL thickness ratio between the THX-B-treated eye and the vehicle control eye. Paired eyes are individually represented. (d) P18 RhoP mice were subconjunctivally injected with THX-B in one eye and with vehicle in the contralateral eye and analyzed at P24. Representative cryosections stained with toluidine. (e) Comparison of the density of nuclei in the ONL between the THX-B-treated eye and the vehicle control eye. Paired eyes are individually represented. Bars represent the mean±S.E.M.; n≥3; **P<0.01; *** P<0.001. Scale bar: 20 μm. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer
