Figure 1 | Cell Death & Disease

Figure 1

From: Conservation and divergence of mitochondrial apoptosis pathway in the Pacific oyster, Crassostrea gigas

Figure 1

Analysis of apoptosis, mitochondrial membrane potential (MMP), and cytochrome c release in UV-irradiated hemocytes of C. gigas. (a) Oyster hemocytes were irradiated with UV light and apoptosis was monitored by Annexin V/propidium iodide (PI) immunostaining at 20 h post irradiation (hpi). Data are shown as the mean±S.D. (N=6). **P<0.01. (b) Oyster hemocytes were UV-irradiated or treated with 10 μM carbonylcyanide-p-chlorophenyl hydrazone (CCCP), and hemocyte MMP was measured by JC-1 assay. Irradiated cells were sampled and measured at 6 hpi, 9 hpi, and 24 hpi. CCCP-treated and untreated/non-irradiated cells served as positive and negative controls, respectively. Lower 590/530 nm ratios refer to lower ΔΨm. N=6 per time point (including negative control and CCCP group), data are shown as the mean±S.D. (c) Cytosolic proteins were extracted from irradiated (24 hpi) and non-irradiated oyster hemocytes and analyzed by western blot using anti-cytochrome c and anti-actin antibodies

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