Figure 3
Sequence analysis of oyster Bcl-2 family proteins. (a) Small motif architecture of Bcl-2 family proteins in Crassostrea gigas. C. gigas Bcl-2 family proteins contained at least three of four conserved sequence motifs known as Bcl-2 homology domains (BH1–BH4, indicated in the figure). Cg-Bak and Cg-Bax also contained the transmembrane domain (gray box). (b) Neighbor-joining phylogenetic tree of Bcl-2 family homologs from different vertebrate and invertebrate species. The neighbor-joining tree constructed by the MEGA program was based on the sequences of four Bcl-2 family proteins in C. gigas, along with Bcl-2 family homologs from other species, including Bcl-2 homologs from Homo sapiens (NP_000624.2), Xenopus laevis (NP_001139565.1), Danio rerio (NP_001025424.1 and NP_571882.1), Drosophila melanogaster (NP_523702.1), Caenorhabditis elegans (NP_499284.1), Lottia gigantea (XP_009061217.1 and XP_009067239.1), Schistosoma japonicum (CAX69465.1), and Hydra vulgaris (NP_001274311.1); Bcl-xL homologs from Homo sapiens (CAA80661.1), Xenopus laevis (NP_001082147.1), and Danio rerio (NP_571882.1), Bak homologs from Homo sapiens (NP_001179.1), Xenopus laevis (NP_001089587.1), Lottia gigantea (XP_009064284.1), Schistosoma japonicum (CAX70134.1), Schistosoma mansoni (CCD80772.1), Schmidtea mediterranea (AEX93474.1, AEX93475.1 and AEX93476.1), and Hydra vulgaris (NP_001296708.1), and Bax homologs from Xenopus laevis (NP_001079104.1), Danio rerio (NP_571637.1), and Schistosoma mansoni (CCD81694.1)
