Figure 1
From: RIP3 attenuates the pancreatic damage induced by deletion of ATG7
Atg7 depletion reduces autophagy activity. (a) Atg7 quantitation and representative IF/scattergram microphotographs of Atg7F/F (n=4) and Atg7Δpan (n=8) pancreatic tissue stained for DAPI (blue) and Atg7 (green) (anti-Atg7 sc-33211, 1/50, scale bar=50 μm). (b) Evaluation of Atg7F/F (n=4) and Atg7Δpan (n=4) pancreatic Atg7 and Atg5 level by immunoblot analysis using the ratio of Atg7/Atg5 and Erk2 (anti-Atg7 sc-33211, 1/200 dilution, anti-Erk2 sc-154, 1/200). (c) Evaluation of Atg7F/F (n=4) and Atg7Δpan (n=4) of LC3-1 to LC3-II, by WB analysis with either the ratio of LC3-II and LC3-I and LC3-II and Erk2 (anti-LC3 Nanotools 5F10, 1/200). (d) p62 quantitation and representative IF/scattergram microphotographs of Atg7F/F (n=4) and Atg7Δpan (n=4) pancreatic tissue stained for DAPI (blue) and p62 (green)(anti-p62 sc-25575, 1/50, scale bar=50 μm). Data are mean±S.E.M. for the numbers of animals as indicated in the graph, *P<0.05, **P<0.01
