Figure 6

High glucose induces apoptosis by regulating MIAT/DAPK2 pathway. (a,b) Cardiomyocytes were transfected with MIAT-siRNA and/or pcDNA-DAPK2 before high glucose (30 mmol/l) exposure and the expression of DAPK2 was analyzed by real-time PCR and western blot. (c) Cardiomyocytes were stained with Annexin V/PI and the apoptotic rate was determined by flow cytometry. *P<0.05. (n=3 independent experiments)