Figure 1
From: Aryl hydrocarbon receptor activation restores filaggrin expression via OVOL1 in atopic dermatitis
OVOL1 regulated FLG expression in NHEKs. (a and b) Data are expressed as mean±S.E.M.; n=3 for each group; *P<0.05. (a) OVOL1 was overexpressed in NHEKs (OVOL1 OE cells) by electroporation of the plasmid containing an open reading frame of human OVOL1. FLG expression in the OVOL1 OE cells was analyzed by qRT-PCR. (b) OVOL1 was knocked down by transfection of OVOL1 siRNA into NHEKs (OVOL1 siRNA cells). FLG expression in OVOL1 siRNA NHEKs was analyzed by qRT-PCR. Mock-transfected NHEKs (c) and OVOL1 OE NHEKs (d) were stained with an anti-FLG antibody (primary antibody) and an Alexa Fluor 546-conjugated anti-mouse IgG antibody (secondary). The nuclei were counterstained with DAPI (blue). Confocal laser scanning images revealed increased FLG expression (red) in OVOL1 OE NHEKs compared with mock-transfected NHEKs. (e) Isotype negative control. The scale bar is 25 μm. The data are representative of experiments repeated three times with similar results
