Figure 5
From: Aryl hydrocarbon receptor activation restores filaggrin expression via OVOL1 in atopic dermatitis
FICZ and Glyteer reversed the IL-4-induced decrease in FLG expression, which was dependent on OVOL1. Control siRNA- or OVOL1 siRNA-transfected NHEKs were treated with FICZ (100 nM) (a) or Glyteer (0.001%) (b) with or without IL-4 (10 ng/ml) for 24 h and then mRNA or total protein of the NHEKs were extracted. Expression of FLG in the NHEKs was analyzed by qRT-PCR. Data are expressed as mean±S.E.M.; n=3 for each group; *P<0.05. (c) Expression of FLG was analyzed by western blotting using the anti-FLG antibody. The data are representative of experiments repeated three times with similar results. The ability of FICZ or Glyteer to reverse the IL-4-induced downregulation of FLG was abrogated in OVOL1-knockdown NHEKs
