Figure 6
KLF5 expression suppresses ccRCC metastasis in vivo. GFP-luciferase (GFP-Luc) lentivirus was infected into NC and KLF5-transduced 786-O cells. These cells were then intravenously injected into nude mice. (a) Western blots were applied to detect expression of KLF5 in GFP-Luc cells. (b–i) Twelve weeks after injection, these mice were euthanized, and BLI images (b) and quantification of photon fluxes (c) or fluorescence images (d) and quantification of fluorescence fluxes (e) or images (f) of lungs were shown and compared. Black arrows in (f) indicated the metastatic nodules. Images at right are magnified view of yellow line circled images at left. The number of macroscopic lung metastasis nodules (g) and lung weight (h) were counted and compared. (i) Representative images of H&E staining of lung metastasis nodules (rounded by yellow lines) were shown. Scale bar, 50 μm. All bar graphs are plotted as mean±S.D. *P<0.05; **P<0.01; ***P<0.001 by two-tailed Student’s t-test
