Figure 1

miR-323a-3p is frequently downregulated and modulated by the methylation of IG-DMR. (a) The relative expression of miR-323a-3p was significantly decreased in three BCa cell lines (T24, UM-UC3, 5637) compared with the normal bladder cell line (SV-HUC-1). (b) The relative expression levels of miR-323a-3p in individual nine pairs of BCa tissues were presented as the fold change of miR-323a-3p referred to the corresponding adjacent normal tissues. (c) The CISH results of TMAs indicated that miR-323a-3p was significantly downregulated in BCa tissues than adjacent non-tumor tissues. (d) The representative images of CISH of TMAs. (e) Downregulation of miR-323a-3p was significantly associated with poor overall survival rate of BCa. (f) An obvious upregulation of miR-323a-3p in T24, UM-UC3 and 5637 cell lines after the treatment with demethylation agent 5-aza-CdR. (g) The location of miR-323a-3p within the DLK1-DIO3 genomic imprinted miRNA cluster and CpG islands region of IG-DMR analyzed by BSP were all presented. (h) Methylation profile of UM-UC-3, T24 and 5637 cell lines. The open and filled circles represent the unmethylated and methylated CpG islands respectively. Five clones from each cell line were analyzed. Error bars represent the S.E. obtained from three independent experiments; *P<0.05