Figure 3 | Cell Death & Disease

Figure 3

From: MET/SMAD3/SNAIL circuit mediated by miR-323a-3p is involved in regulating epithelial–mesenchymal transition progression in bladder cancer

Figure 3

Both upregulated MET and SMAD3 in BCa are direct targets of miR-323a-3p. (a) A qRT-PCR assay detected the significant downregulation of MET, SMAD2, SMAD3 and STAT3 in T24 and UM-UC3 cell lines transfected with miR-323a-3p mimics. (b) A dual-luciferase reporter assay indicated miR-323a-3p significantly suppressed the luciferase activity of vectors that carried 3′-UTRs of MET and SMAD3 but not SMAD2 and STAT3. (c) The targeting sites of miR-323a-3p in the 3′-UTRs of MET and SMAD3 were mutated as represented. (d) Western blot analysis verified that miR-323a-3p repressed the expression of MET and SMAD3. (e) SMAD3 was significantly upregulated in BCa tissues than adjacent non-tumor tissues. (f) Representative images of IHC staining of SMAD3 in TMAs. (g) Kaplan–Meier survival analysis showed that the level of SMAD3 was not associated with overall survival rate of BCa. Error bars represent the S.E. obtained from three independent experiments; *P<0.05

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