Figure 5 | Cell Death & Disease

Figure 5

From: MET/SMAD3/SNAIL circuit mediated by miR-323a-3p is involved in regulating epithelial–mesenchymal transition progression in bladder cancer

Figure 5

The knockdown of SMAD3 repressed the motility of T24 and UM-UC3 cell lines and inhibition of miR-323a-3p expression partially rescues si-SMAD3-induced suppression of cell motility. (a) Trans-well assay suggested the significant inhibition of motility, and the relative migration and invasion rates were calculated. (b) A wound healing assay revealed the consistent repression of the motility of T24 cell line. (c and d) Three SMAD3 siRNAs were merged together as siRNA-pool to transfect T24 and UM-UC3 cell lines and western blot assay revealed inhibition of EMT associated proteins and SNAIL protein. (e,f) Co-transfection of miR-323a-3p-Inh and si-SMAD3 attenuated the expression repressed by si-SMAD3 both at the mRNA and protein levels in T24 and UM-UC3 cell lines. (g) Trans-well assays consistently indicated co-transfection of miR-323a-3p and si-SMAD3 abrogated the motility of T24 and UM-UC3 cell lines. And the relative migration and invasion rate was calculated. Error bars represent the S.E. obtained from three independent experiments; *P<0.05. Photographs of the trans-well assay were obtained under × 10 objective

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