Table 1 Primers, probes and amplicons for quantitative PCR assays
Target | Forward primer (5′→3′) | Reverse primer (5′→3′) | UPL probe | Amplicon (bp) | Intron (bp) | |
|---|---|---|---|---|---|---|
Fas | ENSMUSG00000024778 | TGCAGACATGCTGTGGATCT | CTTAACTGTGAGCCAGCAAGC | #34 | 60 | 16 377 |
FasL | ENSMUSG00000000817 | ACCGGTGGTATTTTTCATGG | AGGCTTTGGTTGGTGAACTC | #21 | 117 | 791 |
c-IAP1 | ENSMUSG00000057367 | GAAGAAAATGCTGACCCTACAGA | CATGACGACATCTTCCGAAC | #80 | 72 | 4393 |
c-IAP2 | ENSMUSG00000032000 | GGGGACGATTTAAAGGTATCG | TCGGTTTTACTGCTAGGCTGA | #5 | 139 | 11 412 |
XIAP | ENSMUSG00000025860 | GCTTGCAAGAGCTGGATTTT | TGGCTTCCAATCCGTGAG | #25 | 88 | 1334 |
p53 | ENSMUSG00000059552 | ATGCCCATGCTACAGAGGAG | AGACTGGCCCTTCTTGGTCT | #78 | 74 | 585 |
Bcl-2 | ENSMUSG00000057329 | GTACCTGAACCGGCATCTG | GGGGCCATATAGTTCCACAA | #75 | 76 | 168 904 |
Bcl-xL | ENSMUSG00000007659 | TGACCACCTAGAGCCTTGGA | TGTTCCCGTAGAGATCCACAA | #2 | 68 | 47 142 |
GAPDH | ENSMUSG00000057666 | GGGTTCCTATAAATACGGACTGC | CCATTTTGTCTACGGGACGA | #52 | 112 | 240 |
