Figure 5
MiR-219 induces neural development in mouse embryos. MiR-219 agomirs, Foxj3/Zbtb18 mRNAs, and their corresponding negative controls were microinjected into the cytoplasm of zygotes as indicated. The expression patterns of Oct4 and Nestin in the serially sectioned E6.5 and E7.5 embryos were examined by immunohistochemistry analysis. (A) Immunohistochemistry analysis of the E6.5 embryos. Representative images are shown. Black dashed frames in E6.5 embryos indicate the embryonic region. (B) Immunohistochemistry (a–d) and H&E staining (e, f) analysis of E7.5 embryos. a and b show the representative control embryo and indicate how the embryonic region is organized and how the ectodermal and endodermal tissues can be distinguished. c and d show the representative abnormal miR-219-injected embryo, which exhibits a severe overdevelopment in its anterior region. (e, f) show representative abnormal Foxj3/Zbtb18 mRNA-injected embryo with disorganized embryonic region, apparent arrested development, or resorption. Full images of serially sectioned embryos are shown in Supplementary Figure S4. a, anterior; ac, amniotic cavity; al, allantois; d, distal; exc, exocoelomic cavity; p, posterior; px, proximal. Scale bars, 200 μm
