Figure 6
Olig1, Zic5, Erbb2, and Olig2 participate in neural regulatory networks. (a) Co-expression networks of differentially expressed genes in the control, Foxj3-ES, and Zbtb18-ES groups. The overlapping genes were selected to construct gene co-expression networks. Sixteen genes located in the four focused GO terms are arrow-shaped. Solid lines, positively corrected; dashed lines, negatively corrected. (b, c) The genes with difference degrees above eight between Foxj3-ES (b) or Zbtb18-ES (c) and control ESCs. (d) The relative levels of Olig1, Zic5, Erbb2, and Olig2 in the ESCs were detected through qRT-PCR. The ESCs were treated or transfected with RA, pCMV-Foxj3, pCMV-Zbtb18, or miR-219 for 48 h. (e–g) Relative levels of Nestin and Map2 were detected through qRT-PCR (e) and western blot (f) in ESCs transfected with Olig1-, Zic5-, Erbb2-, or Olig2-encoding plasmids for 48 h. Relative intensities of protein bands (g) were quantified by using Image J software and calculated by using the samples normalized to β-actin. All data are presented as mean±S.D. and derived from three independent experiments. *P<0.05; **P<0.01; NS, no significance
