Figure 2

Inhibition of autophagy protects astrocytes against OGD injury. Astrocytes were treated with 3-MA (0.1, 0.5 or 1 mM) or Wort (25, 50 or 100 nM), then underwent OGD treatment for 12 h. (a and b) Representative light microscope images of astrocytes without OGD or with OGD treatments. (c and d) LDH leakage analysis showed that 3-MA (c) or Wort (d) treatment decreased the LDH leakage of astrocytes with OGD treatment. Means±S.D., n=6. ##P<0.01 versus non-OGD group; *P<0.05, **P<0.01 versus OGD group