Figure 3 | Cell Death & Disease

Figure 3

From: Inhibition of autophagy blocks cathepsins–tBid–mitochondrial apoptotic signaling pathway via stabilization of lysosomal membrane in ischemic astrocytes

Figure 3

Inhibition of autophagy blocks OGD-induced activation of cathepsin B or cathepsin L–tBid–mitochondrial apoptotic signaling pathway in astrocytes. 3-MA (0.1, 0.5 or 1 mM), Wort (25, 50 or 100 nM) or z-VAD (25, 50 or 100 μM) was added in cells 30 min, 2 h or 1 h before OGD, respectively. (a-j) Representative western blotting images of the protein levels of LC3-II (a and b), active cathepsin B (c and d) at 6 h or cathepsin L (e and f) at 3 h, tBid (g), mitochondrial (h) and cytoplastic Cyt-c (i) and active caspase-3 (j) at 12 h after OGD. (l-u) Columns represent quantitative analysis of immunoblots in (a-j), respectively (means±S.D., n=3). COX IV acts as a mitochondrial marker. β-Actin or HSP-60 was used as a loading control. ##P<0.01 versus non-OGD group; *P<0.05, **P<0.01 versus OGD group

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