Figure 5 | Cell Death & Disease

Figure 5

From: Inhibition of autophagy blocks cathepsins–tBid–mitochondrial apoptotic signaling pathway via stabilization of lysosomal membrane in ischemic astrocytes

Figure 5

Inhibition of autophagy reduces OGD-induced release of cathepsin L in astrocytes. (a) The cells were treated with OGD for 3 h, and 3-MA (1 mM) or Wort (100 nM) was added in cells 30 min or 2 h before OGD, respectively. Then double immunofluorescence staining of cathepsin L (red) and Lamp 1 (green) was performed by corresponding antibodies. Hoechst (blue) was used to stain nuclei. Images were captured by a confocal microscopy. Magnified images (M) were cropped sections from the merge images (white borders). (b) Quantification of red fluorescence intensity of cathepsin L immunostaining in (a). (c) PCC and MOC demonstrated colocalization between cathepsin L and Lamp 1. Image-Pro Plus was used to calculate the colocalization coefficients. Means±S.D., n=6. ##P<0.01 versus non-OGD group; **P<0.01 versus OGD group

Back to article page