Figure 7 | Cell Death & Disease

Figure 7

From: Inhibition of autophagy blocks cathepsins–tBid–mitochondrial apoptotic signaling pathway via stabilization of lysosomal membrane in ischemic astrocytes

Figure 7

Inhibition of autophagy decreases LMP in OGD-treated astrocytes with AO-uptake and Lyso-Tracker Red uptake methods. (a and b) Representative photomicrographs of AO staining (a) or Lyso-Tracker Red staining (b). Cells were treated with OGD for 6 h, and then incubated with AO (5 μg/ml) for 15 min or Lyso-Tracker Red (75 nM) for 60 min. 3-MA (1 mM) or Wort (100 nM) was added in cells 30 min or 2 h before OGD, respectively. The pictures were captured by a confocal microscope. Magnified images (d', e', f', j', k', l', p', q', r', v', w', x') were cropped sections from the white borders areas in the images (a', b', c', g', h', i', m', n', o', s', t', u'), respectively. (c and d) Quantification of red fluorescence intensity of AO staining (c) or Lyso-Tracker Red staining (d). Means±S.D., n=6.##P<0.01 versus non-OGD group; **P<0.01 versus OGD group

Back to article page