Figure 6
From: Binding of doxorubicin to Sorcin impairs cell death and increases drug resistance in cancer cells
Sorcin silencing increases doxorubicin accumulation in H1299 cells. (a) confocal microscopy images, showing the nuclear accumulation of doxorubicin upon 3 h treatment in H1299 cells treated with scrambled siRNA (top) or with Sorcin siRNA (bottom); (b) nuclear doxorubicin incorporation by fluorescence quantification (n=60 cells; P<0.0001); (c) time-dependent quantification of doxorubicin incorporation in H1299 cells by FACS; (d) upon 48 h doxorubicin treatment, in Sorcin-silenced cells PARP cleavage is increased with respect to control cells (n=3, P<0.01). Sorcin silencing decreases MDR1 activity and expression with respect to control cells: (e) Rhodamine123 fluorescence is increased (and therefore its efflux is decreased) and (f) MDR1 expression is decreased in Sorcin-silenced H1299 cells with respect to control cells (n=3, P<0.001)
