Figure 7

Hypertonicity-induced TNF release overcomes resistance to SM monotherapy. (a) TF-1 cells were challenged in the presence and absence of 50 mM NaCl with the indicated concentrations of birinapant. Shown are data points and mean±S.E.M. from three independent experiments. (b) Co-cultures of TF-1 and REH cells under iso- and hypertonic (+50 mM NaCl) conditions were challenged with the indicated concentrations of birinapant in the presence and absence of etanercept (1 mg/ml). Shown are data points and mean±S.E.M. from three independent experiments. (c) TF-1 and REH cells were challenged with 10 μM birinapant in the presence of 50 mM NaCl for 18 h or left untreated. Supernatant of REH cells was harvested and transferred onto TF-1 cells. After 7 h, viability of TF-1 cells was measured using MTT. Shown are data points and mean±S.E.M. from four independent experiments. Biri, birinapant; SN, supernatant