Figure 3

Onset of puberty in WT and Bmf^−/− female mice. (a) The percentages of WT and Bmf^−/− mice with at least one corpus luteum present in their ovaries were determined at PN20, 30, 40 and 50 (n=6/age/genotype). The numbers of mice analysed are shown above each bar. (b) The average numbers of corpora lutea present per ovary in WT and Bmf^−/− mice (only mice with at least one corpora lutea were included in the analysis) were determined at PN20, 30, 40 and 50. Data are expressed as mean±S.E.M. (where n=3 or more). ^#P<0.05 for comparison of Bmf^−/− versus WT at PN50 (two-tailed unpaired student’s t-test). (c) Representative images of PAS-stained ovarian sections from WT and Bmf^−/− mice at PN20, 30, 40 and 50. The notation ‘CL’ indicate the location of each corpus luteum. Scale bars=200 μm