Figure 1 | Cell Death & Disease

Figure 1

From: MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells

Figure 1

TRAIL-R2 is a direct target of miR-519a-3p. (a) qRT-PCR reveals downregulation of TRAIL-R2 (TR2), but not TRAIL-R1 (TR1) and Fas in MCF10A, MDA-MB-231, HCC1143, T47D and MDA-MB-468 cells. Cells were transfected with miRNA control or miR-519a-3p for 48 h, mRNA was isolated and gene expression of TRAIL-R1, TRAIL-R2 and Fas was analyzed (n=3). (b) Schematic representation of the miR-519a-3p target sites within the 3′UTR of TNFRSF10B (TRAIL-R2) mRNA. (c) Luciferase reporter assays were performed using psiCHECK constructs in MCF-7 cells. Luciferase activity of TNFRSF10B 3′UTR is reduced after miR-519a-3p transfection compared with control. The signal of the wt UTR (TRAIL-R2-wt) is rescued after mutating all three binding sites for miRNA-519a-3p (TRAIL-R2-trip.mut) (n=6). Data are expressed as mean+S.D.; **P<0.01, ***P<0.001. All P-values are based on analysis of miRNA control versus miR-519a-3p

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