Figure 2 | Cell Death & Disease

Figure 2

From: MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells

Figure 2

MiR-519a-3p downregulates caspases-7 and -8 at RNA and protein levels. (a) qRT-PCR reveals downregulation of CASP7 and CASP8 by miR-519a-3p in MCF10A, MDA-MB-231, T47D, HCC1143 and MDA-MB-468 cells (n=3). (b) Western blot analysis confers qRT-PCR results showing downregulation of caspases-7 and -8 by miR-519a-3p (miR-519a). β-Actin was used as a loading control. (c) Schematic representation of the miR-519a-3p target sites within the 3′UTR of caspase-7 and caspase-8 mRNA. (d) Luciferase activity is reduced after miR-519a-3p transfection compared with control with the wild-type CASP8 (CASP8-wt) but not with the CASP7 (CASP7-wt) 3′UTR in MCF10A cells. The signal is rescued after mutating binding site for miRNA-519a-3p (CASP8-mut) (n=6). Data are expressed as mean+S.D.; *P<0.05, **P<0.01, ***P<0.001. All P-values are based on analysis of miRNA control versus miR-519a-3p

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