Figure 10 | Cell Death & Disease

Figure 10

From: Klotho enhances FoxO3-mediated manganese superoxide dismutase expression by negatively regulating PI3K/AKT pathway during tacrolimus-induced oxidative stress

Figure 10

Klotho reduces Tac-induced apoptosis in HK-2 cells. HK-2 cells were seeded in culture plates at 90% confluence. On next day, the cells were treated with Tac (60 μg/ml) in the absence or presence of 1 μg/ml rKlotho and 25 μM LY294002 (LY, PI3K inhibitor) for 12 h. Before the end of the treatment, annexin V solution was added to each well for 15 min to measure apoptosis, which was analyzed by flow cytometry. Whole-cell lysates were collected after each 12-h drug treatment to measure the relative protein expression levels of Bcl-2, Bax, active caspase-9 and active caspase-3. (a and b) Flow cytometry histograms and a graph of annexin V labeling. (c) Representative western blots for Bcl-2, Bax, active caspase-9, and active caspase-3. All proteins are normalized to β-actin expression. LY; LY294002. The data are presented as the mean±S.E. #P<0.05 versus the VH group; $P<0.05 versus the rKlotho groups; @P<0.05 versus the Tac group; !P<0.05 versus the Tac+rKlotho group

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