Figure 6 | Cell Death & Disease

Figure 6

From: Klotho enhances FoxO3-mediated manganese superoxide dismutase expression by negatively regulating PI3K/AKT pathway during tacrolimus-induced oxidative stress

Figure 6

Klotho induces FoxO3a binding to the MnSOD promoter in Tac-treated HK-2 cells. HK-2 cells were seeded in culture plates at 90% confluence. On the next day, the cells were treated with Tac (60 μg/ml) in the absence or presence of 1 μg/ml rKlotho. After 12 h, the cells were harvested to detect MnSOD transcription activity after nuclear FoxO3a translocation using a ChIP assay and quantitative real-time PCR (q-PCR) for MnSOD expression. (a) The FoxO3a protein/MnSOD promoter complex was analyzed by q-PCR with primer pairs for the MnSOD promoter region containing a FoxO3a-binding element. (b) MnSOD mRNA was detected by q-PCR in whole-cell lysates. Relative MnSOD expression was presented after normalization to cyclophilin A expression. The data are presented as the mean±S.E. #P<0.05 versus the control group; $P<0.05 versus the rKlotho groups; @P<0.05 versus the Tac group

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