Figure 7 | Cell Death & Disease

Figure 7

From: Klotho enhances FoxO3-mediated manganese superoxide dismutase expression by negatively regulating PI3K/AKT pathway during tacrolimus-induced oxidative stress

Figure 7

Klotho reduces Tac-induced mitochondrial ROS production and mitochondrial damage in HK-2 cells. HK-2 cells were seeded in culture plate at 90% confluence. On the next day, the cells were treated with Tac (60 μg/ml) in the absence or presence of 1 μg/ml rKlotho and 25 μM LY294002 (LY, PI3K inhibitor) for 12 h. The cells were exposed in MitoSOX or MitoTracker, then analyzed by flow cytometry and confocal microscopy. MitoSOX staining to detect mitochondrial superoxide anion (O2−) levels by confocal microscopy (a) or flow cytometric analysis (b and c). MitoTracker staining to detect the number of mitochondria by confocal microscopy (d). (e) Relative fluorescence intensity of MitoTracker staining by flow cytometry. LY; LY294002. Scale bar=20 μm. The data are presented as the mean±S.E. #P<0.05 versus the VH group; $P<0.05 versus the rKlotho groups; @P<0.05 versus the Tac group; !P<0.05 versus the Tac+rKlotho group

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