Figure 1
From: PDI is an essential redox-sensitive activator of PERK during the unfolded protein response (UPR)
Simultaneous knockdown of ERp57 and PDI (DK) in HCT116 cells reduces apoptosis as compared with ERp57 knockdown alone. Every experiment was repeated twice and within the experiments at least three samples were treated in the same way. Bars represent mean±S.D. Two groups were analyzed by Student’s t-test, and more than two groups by ANOVA with post hoc Tukey’s test. Statistical significance is presented as **P<0.01. (a) At 96 h after KD induction and 48 h after irradiation with 10 Gy, caspase-3 activity was measured in whole-cell lysates of shERp57 and DK. Annexin/PI staining at 96 h after KD induction was used to validate the caspase-3 activity assays. (b) Representative western blots displaying KD efficiency of ERp57 and PDI and protein levels of p53. (c) At 96 h after KD induction, PARP cleavage of DK and shERp57 cells was shown by western blot. (d) Apoptosis levels in DK p53−/− cells measured by caspase-3 activity assay. Representative western blot shows KD efficiency and p53 deficiency in p53−/− cells
