Figure 3
From: PDI is an essential redox-sensitive activator of PERK during the unfolded protein response (UPR)
Simultaneous depletion of ERp57 and PDI does not activate the UPR sensors ATF6 and IRE1. Every experiment was repeated twice and within the experiments at least three samples were treated in the same way. Bars represent mean±S.D. (a) Confocal microscopy images of HCT116 WT or DK cells transfected with an ATF6-GFP fusion construct. The 2 μM thapsigargin (Thaps) was used as positive control. (b) ATF6 reporter gene assay, 10 μM Thaps was used as positive control. (c) Western blot showing KD efficiency and the ATF6 target gene BiP. (d) At 48 h after irradiation and 96 h after KD induction, RT-PCR was performed to detect the spliced form of XBP1 as an indicator for IRE1 activation. (e) XBP1 reporter gene assay 96 h after KD induction
