Figure 4
From: PDI is an essential redox-sensitive activator of PERK during the unfolded protein response (UPR)
The UPR sensor PERK is activated in HCT116 shERp57 cells and contributes to apoptosis induction. KD was induced for 96 h. Every experiment was repeated twice and within the experiments at least three samples were treated in the same way. Bars represent mean±S.D. Statistical significance is presented as **P<0.01. (a and b) Comparison of PERK phosphorylation, eIF2α phosphorylation and ATF4 expression in shERp57 and DK cells by western blot. (c) Luciferase assay performed with an Nrf2-responsive element plasmid in DK, shERp57 and shPDI cells. (d) Caspase-3 activity assay in shERp57, shPDI and DK cells after 48 h of PERK inhibition with GSK2606414. (e) HCT116 shERp57 und DK were irradiated with 10 Gy 48 h after KD induction. After 48 h, cell cycle distribution was determined by PI staining and subsequent FACS analysis
