Figure 6
From: PDI is an essential redox-sensitive activator of PERK during the unfolded protein response (UPR)
PDI is required for sufficient and cytoprotective PERK activation under global ER stress. Every experiment was repeated twice and within the experiments at least three samples were treated in the same way. Bars represent mean±S.D. (a) HCT116 shPDI cells were treated with 2 μM thapsigargin for 20 h and used for caspase-3 assay. Corresponding phase contrast microscopy pictures are shown. (b) Western blot shows PARP cleavage after 24 h of treatment with thapsigargin (2 μM) or bortezomib (0.5 μM) and 96 h KD in shPDI cells. (c) Western blot of shPDI cells treated with 2 μM thapsigargin. PERK, P-eIF2α, ATF4 GRP94, PARP, BiP and PDI are shown. (d) shPDI- or PACMA31-treated HCT116 cells were cultured under 21% or 1% oxygen. Phase contrast microscopy and caspase-3 assays are shown
