Figure 1
The histological characteristics and expression levels of miR-30a-e in rat renal proximal tubules treated with cisplatin. (a–d) Representative images of H&E-stained rat renal tubules are taken from the kidneys of untreated rats (a) and those treated with 10 mg/kg cisplatin for 1 day (b), 10 mg/kg cisplatin for 3 days (c) and 10 mg/kg cisplatin for 7 days (d). (a1–d1) The higher-magnification images focus on renal proximal tubules from a to d, respectively. (e) The western blotting data show the protein expression of cleaved caspase3 in rat renal tissue from the untreated and the 1-, 3- and 7-day cisplatin-treated groups. In addition, the bar chart shows the comparison of the relative expression of cleaved caspase3 (caspase3/tubulin) between the control group and the groups treated with cisplatin for different durations. (f) Representative TUNEL immunohistochemistry was implemented on kidney transverse sections from the untreated and the 1-, 3- and 7-day cisplatin-treated groups. Arrows indicate TUNEL-stained apoptotic nuclei of the tubular epithelial cells. (g) The bar chart shows the ratios of TUNEL-positive cells divided by total cells from 10 microscopic fields at × 400 magnification. (h) Caspase activity is measured by enzymatic assays using DEVD.AFC acts as the substrate. The bar chart shows caspase activity of cisplatin-treated groups over untreated group. (i) The bar chart shows the quantitative PCR data on the expression of miR-30a, miR-30b, miR-30c, miR-30d and miR-30e in the normal rat renal tissue. (k) The bar chart shows the quantitative PCR data in the expression of miR-30a, miR-30b, miR-30c, mR-30d and miR-30e in the rat renal tissue from the untreated and cisplatin-treated groups treated for 1, 3 and 7 days. Scale bars=200 μm in a–d and a1–d1. Data represent the mean of three independent experiments±S.E.M. with n=5. *P<0.05; **P<0.01; ***P<0.001
