Figure 2
The assessment of apoptosis and the expression levels of miR-30a-e in HK-2 and NRK-52E cells treated with cisplatin. (a and b) HK-2 (a) or NRK-52E cells (b) were treated with 10 μM cisplatin for different times. After treatment, HK-2 (a, upper panel) or NRK-52E (b, upper panel) cells were stained with Annexin V–FITC and PI for determining cell apoptosis using flow cytometry assay. The bar chart (right) shows the ratios of apoptosis cell numbers between the control and cisplatin-treated groups. The apoptosis of HK-2 (a, lower panel) and NRK-52E (b, lower panel) cells was also examined by TUNEL assay. The bar chart (right) shows the ratios of TUNEL-positive cell divided by total cells from 10 microscopic fields at × 400 magnification between the control and cisplatin-treated groups. (c and d) The western blotting data show the protein expression of cleaved caspase3 in HK-2 (c) and NRK-52E cells (d) from the control and cisplatin-treated groups. In addition, the bar charts (right) show the comparison of the relative expression of cleaved caspase3 (caspase3/tubulin) between the control and cisplatin-treated groups. (e and f) The bar chart shows the quantitative PCR data on the expression of miR-30a, miR-30b, miR-30c, miR-30d and miR-30e in the HK-2 (e) and NRK-52E cells (f). (g and h) The bar chart shows the quantitative PCR data on the expression of miR-30a, b, c, d and e in the HK-2 (g) or NRK-52E cells (h) of the control and cisplatin-treated groups. Data represent the mean of three independent experiments±S.E.M. with n=3. *P<0.05; **P<0.01; ***P<0.001
