Figure 6 | Cell Death & Disease

Figure 6

From: MiR-30c regulates cisplatin-induced apoptosis of renal tubular epithelial cells by targeting Bnip3L and Hspa5

Figure 6

MiR-30c directly targets Bnip3L and Hspa5. (a and b) The sequence alignment between miR-30c and the predicted binding sites or mutational site in the 3′-UTR of Bnip3L and Hspa5. (c and d) Firefly luciferase reporters containing either wild-type (WT) or mutant (Mut) Bnip3L or Hspa5 3′-UTR were co-transfected into HK-2 cells with the miR-30c mimic, negative control (NC), miR-30c inhibitor or NC inhibitor. Forty-eight hours after transfection, cells were assayed for firefly luciferase and Renilla luciferase assays. Data represent the mean of three independent experiments±S.E.M. with n=4. *P<0.05; **P<0.01

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