Figure 1

CD45RA⁻CCR7⁻ effector/memory Treg subset constituted the majority of Tregs and accumulated in GC. (a) Treg percentage in CD4⁺ T cells in each tissue of patients with GC by gating on CD3⁺CD4⁺CD25⁺Foxp3⁺ cells. Cumulative results from 51 GC patients and 45 healthy donors are shown. (b) Dot plots of surface and intracellular molecule staining for Tregs gating on CD4⁺ T cells, and multicolor flow cytometry for markers or subpopulations of intratumoral Tregs. The horizontal bars and each ring in panel b represent mean values and one patient. GITR, glucocorticoid-induced tumor necrosis factor receptor; CTLA-4, cytotoxic T lymphocyte-associated antigen-4. (c) Statistics analysis of CD45RA⁺ and CD45RA⁻ Treg percentage or CCR7⁺ and CCR7⁻ Treg percentage in total Tregs in tumor and non-tumor tissues of GC patients. (d) Statistics analysis of the percentages of CD45RA⁺CCR7⁺, CD45RA⁻CCR7⁺, CD45RA⁻CCR7⁻, and CD45RA⁺CCR7⁻ Treg subsets in total Tregs in non-tumor or tumor tissues. (e) Dot plots of surface staining and pie charts summarizing for CD45RA⁺CCR7⁺, CD45RA⁻CCR7⁺, CD45RA⁻CCR7⁻, and CD45RA⁺CCR7⁻ Treg subsets by gating on total Tregs. (f) The number of CD45RA⁻CCR7⁻ Treg subset per million total cells, or CD45RA⁻CCR7⁻ Treg subset percentage in total Tregs in blood or each tissue of patients with GC by counting or gating on Tregs. The horizontal bars and each ring or dot in panels a, c, d and f represent mean values and one patient. *, P<0.05; **, P<0.01, and n.s., P>0.05 for groups connected by horizontal lines